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Materials and methods The study was conducted in vitro on human LCA cells (A-549) and the study protocol application groups in vivo on Swiss albino mice treated with benzo[a]pyrene only and were not received any treatment for inducing LCA, and only after LCA induction the study treatment protocol began, treatment was daily with CCFHIONP as HIFUSPDT sensitizer with or without exposure to laser (IRL) or high-frequency ultrasound (HIFUUS) or a combination of laser and/or high-frequency ultrasound for 3 min for 2 weeks

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In healthy tissue, the peptide maintains NO homeostasis by modestly increasing endothelial NOS (eNOS) for beneficial vasodilation while suppressing inducible NOS (iNOS) that drives pathological inflammation

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