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Different ablation protocols and parameters have been utilized to optimize this ratio, resulting in varying degrees of post-ablation immunogenic profiles (17, 18)

Mix 2 (10 L of enzyme A and 20 L of Buffer Y, Mitenyi Biotec, #130-092-628) was added to the digestive solution for 1 h from this step

Krutzsch HC

In normal liver, KCs are the predominant liver macrophages, however, when NAFLD occurs, especially in the NASH state, lipotoxic hepatocytes stimulate KCs to release chemokines, such as CCL2, CCL5 and CSCL10, and render the hepatic recruitment of MDMs

This process was mechanistically linked to enhanced DNA damage and the accumulation of dsDNA, which triggered the activation of cGAS-STING signaling