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The transport assay buffer used was HEPES (50 mM, pH 7.4), KCl (50 mM), MgCl 2 (6 mM) and contained ATP or AMP (4 mM) with and without GSH (5 mM)

Epidermal growth factor is a small protein containing three disulfide bonds and has been produced using many different expression systems [29,30,31,32]

doi: 10.1002/mds.25982 196 Serrano-PozoA.DasS.HymanB

Modifications of nanoparticles with chitosan Chitosan is a natural-based polysaccharide loaded with amino chemical moieties that are extensively exploited in biomedicine because it is biocompatible and exhibits bioactivity