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The sections were then washed and incubated with either monoclonal mouse anti-IGF-1 receptors (Sigma, 1:1000) or rabbit anti-phosphorylated IGF-1 receptors (pY1161, Abcam, Sapphire Bioscience (NZ) Ltd., Hamilton, NZ, 1:100) at 4C for 48 hours

Lyophilized peptide powders offer superior stability compared to liquid formulations because the freeze-drying process removes water the primary driver of peptide degradation

doi:10.1016/j.yrtph.2016.03.020

Core histones are key targets, with H2A monoubiquitination essential for neurodevelopment and linked to related disorders (Srivastava et al., 2017

This timing consideration applies to all GHRH analogs