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40 nm diameter may be the optimal size for non-phagocytic uptake (26), while 200 nm may be the optimal size for crossing the blood-brain barrier (27)

The compounds (and the OD 600 nm reached) of these positive hits included Cianidanol (OD 600 nm =1), Juglone (OD 600 nm =0.66), Deoxycholic acid (OD 600 nm =0.89), Carbenoxolone (OD 600 nm =0.58), Gossypin (OD 600 nm =0.77)(Figure S7)

It is essential, however, to note that the clinical applicability of these biomarkers will require thorough validation via additional studies

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Establishment of PDXOS model and therapeutic effect evaluation The osteosarcoma patient-derived xenograft animal model (PDX) was established in female BALB/c nude mice