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Furthermore, the constant degranulation of mast cells depletes the body's supply of the diamine oxidase (DAO) enzyme, which is responsible for breaking down histamine in the digestive tract

Xue et al., 2015)

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After incubation for 3 days at 37 C in a humidified atmosphere of 5% CO 2 , cells were fixed with 0.05% glutaraldehyde, followed by staining with 1% crystal violet in 20% ethanol for plaque counting