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Protein overexpression was induced by adding 0.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG) to bacterial cultures grown at 37C until an OD 600nm of 0.6

During this stage, the transport of wastes and nutrients by the retinal pigment epithelium (RPE) slows down

Honokiol, a polyphenol from Magnolia officinalis Rehder & E.H.Wilson [Magnoliaceae], inhibits SREBP-1c nuclear translocation, effectively reducing hepatic lipid accumulation (Yin et al., 2009)

This dual mechanism is why tirzepatide has produced greater weight loss in clinical trials than single-agonist GLP-1 drugs like semaglutide

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