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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

Furthermore, tamoxifen-induced apoptosis was mediated via cancerous inhibitor of protein phosphatase 2A (CIP2A) /protein phosphatase 2A (PPA2) / phospho-Akt (p-Akt) cascade in MDA-MB-231, MDA-MB-468, MDA-MB-453 and SK-BR-3 cells [89]

Please note: Immediately consult your doctor if you develop fever or the pain and swelling fail to resolve after 48 hours following the injection.

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Improper handling defeats proper bacteriostatic water technique during preparation