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Acute alcohol consumption disrupts redox balance and causes cellular oxidative stress, which is indicated by markers such as MDA and CYP2E1

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After centrifugation, the supernatants were added to 2.3 mL of deionised water, 100 mL of 0.3M EDTA, 300 mL of 0.32M tris(hydroxymethyl)aminomethane (TRIS) and 100 mL of 0.086 mM 5,5-dithiobis-2-nitrobenzoic acid (DTNB), and were maintained at 10 C for 10 min

however, many other proteins remain poorly studied or unidentified, leaving significant gaps in our understanding of their specific roles in oxidative stress tolerance and pathogenesis [3]