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Increased production of ROS increases oxidative stress and therefore promotes apoptosis via multiple caspase pathways in PTECs upon exposure to high glucose (89, 90)

87 These findings clearly indicate that the stimulation of increases in eNOS expression without simultaneously increasing vascular levels of BH 4 may lead to eNOS uncoupling because of the stoichiometric relationship between endothelial BH 4 and NOS activity

Cell culture The following cell lines were obtained from the American Type Culture Collection (ATCC), which authenticated them via short tandem repeat profiling: the human sarcoma cell line U-2 OS (ATCC: HTB-96

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