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Primary patient-derived AML cells were isolated using Ficoll gradients and were cultured in IMDM (Thermo Fisher Scientific, USA) supplemented with 15% BIT 9500 Serum Substitute (STEMCELL Technologies, Canada), 100 ng/ml stem cell factor (SCF) (ImmunoTools, Germany), 50 ng/ml FLT3L (ImmunoTools, Germany), 20 ng/ml interleukin-3 (IL-3) (ImmunoTools, Germany), 20 ng/ml G-CSF (ImmunoTools, Germany), 0.1 mM 2-mercaptoethanol (Gibco, USA), 50 g/ml gentamicin (Thermo Fisher Scientific, USA), 10 g/ml ciproflaxin (Thermo Fisher Scientific, USA), 500 nM SR1 (APExBIO, USA) and 1 M UM279 (APExBIO, USA) at 37 C, 5% CO 2 , and 95% humidity

Xiaojun M, Min S, Yushan Y, Gaofei R, Jingwen H, Guijun Q

The HH equation tells us that this will depend on the pH and the pKa of the functional group

MMHC is the recipient of PhD scholarship from the Faculty of Medicine, Universit de Sherbrooke

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