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FOXO4-DRI administered during this peak window encounters maximum lysosomal activity, enhanced membrane permeability, and upregulated p53 pathway sensitivity

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

its strictly about understanding the mechanism within a controlled research setting