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Coupling efficiency was determined by calculating the proportion of rotenone/antimycin A sensitive oxygen consumption that is also sensitive to oligomycin (Sigma-Aldrich, 75351) treatment

Giustarini, D., Milzani, A., Dalle-Donne, I

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The truncated Syn expressing cells were harvested by centrifugation, and the resulting pellet was resuspended in lysis buffer and sonicated