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A total of 3 sections per block were incubated in DAKO antigen retrieval buffer at pH 9.0 (Agilent, S236784-2) for antigen retrieval and then blocked in 10% goat serum in PBS for 1 hour at 4C

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Cremer DR, Rabeler R, Roberts A, Lynch B (2006) Safety evaluation of -lipoic acid (ALA)

The interplay of these factors underscores the urgent need for climate-resilient agricultural strategies, biofortification efforts, and microbiota-targeted interventions to mitigate the long-term health consequences of climate change on human nutrition and gut health