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ROS staining Wt animals reared on LB or DB

The 30-min gradient elution program was set as follows: an increment from 5% B to 22% B over 20 min, a rise to 37% B for 4 min, a subsequent increase to 80% B for 2 min, followed by an isocratic hold at 80% B for 4 min

Using our protocol we have also prepared the linaclotide drug for chronic constipation and irritable bowel syndrome, made of a 14 AAs peptide and bearing three disulfide bonds (Fig

10.3389/fbioe.2021.736679 [DOI] [PMC free article] [PubMed] [Google Scholar] Bonthula S., Bonthula S