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We observed that in the 5mM 5-OP-treated cells the intracellular levels of 5-OP increased 12-fold, while in 10mM it had increased 20-fold (Fig 1D)

For retrograde tracing from the small intestine to the DMV, FG (fluorochrome 4% in PBS, 10 L) was injected into the small intestine, or scAAV2/Retro-hSyn-Cre-WPRE (20 L) was injected into the small intestinal wall at different sites (200 nL per site) and AAV-DIO-mCherry was injected into the DMV of C57 mice