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when reduced by TRX, Ref-1 increases DNA binding affinity of several TFs, including AP-1, NF-B, ATF/CREB, Myb, and p53 [78]

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The X-ray-derived data of small molecular fragments in complex with TGR used in this study to design the compounds here described are available in the PDB under accession codes 6FTC, 6FMU, 6FMZ, 6FP4, 2X99, 2X8G, 2X8H, 6ZST, 6ZP3, 6ZLP, 6ZLB, 7B02, 7NPX, 2V6O, 6RTJ, 6RTO, 6RTM, and 3H4K

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Normally, NF-B is bound to its inhibitor IkB, thus remaining confined to the cytoplasm: to be activated NF-B is phosphorylated by the IB kinase (IKK) complex, which leads to the IkB degradation and allows the transcription factor translocation into the nucleus [117]