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This water-soluble vitamin is not only involved in neuronal differentiation, maturation, and survival, but also acts as a cofactor in different enzymatic reactions involved in catecholamine production, collagen synthesis, and HIF-1 regulation [72]

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The supernatant was discarded, and the RNA pellet was washed twice with 75% ethanol at 4 C and finally resuspended in RNase-free water

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