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For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium
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Among the synaptic proteins, cell adhesion molecules (neuroligins (NLGNs) 176 and neurexins (NRXNs) 61 ), postsynaptic scaffolding proteins (SH3 and multiple ankyrin repeat domains protein (SHANK), 177 glutamate receptors (NMDAR subunit, GluN2B), 178 inhibitory GABA A receptor subunits 3 and 3 (GABRA3 and GABRB3, respectively) 179 and permeable ion channels (voltage-dependent calcium channel subunit 1C (CACNA1C) 180 and sodium channel protein type 1 subunit- (SCN1A) 181 ) are reported to be important signal transduction molecules associated with ASD
