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Contact: [email protected] or (800) 728-5483 Date: September 29, 2023

doi: 10.1016/j.psj.2019.11.069 33 Al-BatshanHAAl-MufarrejSIHomaidanAAQureshiMA

Heat shock response inhibits nf-b activation and cytokine production in murine kupffer cells

After ~ 24 h of incubation, the culture medium was removed by a gentle swing, and cells were lysed in situ with 20 L assay solution containing 1 AlphaLISA Immunoassay buffer (PerkinElmer AL000F), protease inhibitor cocktail, 15 g/mL anti-Flag acceptor beads (PerkinElmer AL112R), and 11.25 g/mL glutathione donor beads (PerkinElmer 6765301)