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In the pancreatic tumour microenvironment, NGF binds tyrosine kinase A (TrkA) receptors, leading to the activation of various downstream signalling cascades, such as PI3K (phosphatidylinositol 3-kinase)Akt signalling pathway, which contributes to survival of both cancer and neuronal cells 51 , another one is RASMAPK (mitogen-activated protein kinase signalling pathway, which leads to the survival, proliferation and invasion of cancer cells 51

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The tag comprises six or more consecutive histidine amino acid residues positioned at either N or C terminus of a recombinant protein

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