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We found that the GFP-Atg8 processing was minimal after 1 h in SD-N + 3-IAA (Supplementary Fig

(2014) 9:e86813

In further embodiments, the invention encompasses a method of inhibiting NNMT in vitro or in vivo by contacting a cell expressing NNMT with one or more cations chosen from 1c, 1f, 1l, 1m, 2j, 2k, 2l, 2m, 2aa, 1c, 1f, 1l, 1m, 2j, 2k, 2l, 2m, and 2aa