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Klicek R et al

PMCID: PMC8078508 Peng Z, Lu Q, Verma DPS (1996) Reciprocal regulation of Delta(1)-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants

All crystal waters were included and the resulting system was further solvated with a 99.0 rhombic dodecahedron (RHDO) box of TIP3P waters 82 which gives at least 9 of solvation between the edge of the protein to the simulation box boundary along the longest protein axis

Ni-NTA beads were equilibrated in Buffer A and added to the cleared supernatant

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