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Levels of RBC vesicle contamination in the purified platelet fraction were measured by determining the levels of glycophorin A (GPA) positive fragments using an anti-human GPA antibody (BD Bioscience, Pharmagen, San Diago, CA) and analysis by flow cytometry

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No statistically significant effect of Kat6b status on H3K14ac was observed (Supplemental Figure 6, A and B)

however, this study did not analyze whether PP2A binds cytoplasmic and/or nuclear CDK5

Specific primary antibodies were shown in Table 2, and horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (1:4000