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Briefly, a standard curve was generated using known concentrations of bovine serum albumin (Sigma) and used to calculate sample concentrations using a Varioskan Flash plate reader (Thermo Scientific)

Zhao Y, Zhang Y, Kong H, Cheng G, Qu H, Zhao Y (2022) Protective effects of carbon dots derived from Armeniacae semen amarum carbonisata against acute lung injury induced by lipopolysaccharides in rats

Animal care and all experimental procedures were performed in accordance with the Guideline for Animal Experimentation of Nagasaki University with the approval of the Institutional Animal Care and Use Committee (permission number: 1803191437)

Observations of clinical toxicity and mortality signs, including changes in skin, fur, eyes, mucous membranes, occurrence of secretions and excretions and autonomic activity (e.g., lacrimation, piloerection, pupil size, unusual respiratory pattern), were monitored twice per day

They also produce gas and substances that can damage the gut lining