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Proteomic analysis In-solution trypsin digestion: 200 L of solution were mixed with 100 L of 25 mM ammonium bicarbonate buffer pH 8.5, reduced by adding 30 L of 10 mM DTT and incubated at 56 C for 20 min

The CRISPR/Cas9 ALMS1 -encoding plasmids (KN414632, OriGene) were also applied by lipofectamine transfection according to the manufacturers instructions, and cells were analyzed after puromycin (P8833, Sigma) selection

Glucagon resistance at the level of amino acid turnover in obese subjects with hepatic steatosis

FXR-deficient mice exhibit impaired insulin signalling and glucose homoeostasis disorders