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In addition, there is a higher susceptibility of Nrf2 -null mice to liver injury [42], while the semi-synthetic oleanane triterpenoid compounds that activate Nrf2 were shown to be hepatoprotective in different models of liver disease (nonalcoholic fatty liver disease, acetaminophen-induced liver injury, and immune-mediated hepatotoxicity) that have been tested [40, 43,44,45]

The parameters of a six-point calibration curve were used for glutathione concentration determination

The beads were washed four times with high-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 300 mM KCl, and 0.1% Triton X-100), followed by two washes with low-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 100 mM KCl, and 0.1% Triton X-100)

& Rost, J

The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants