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To this end, we cultured regular LAPC4-AD cells long-term (4 months) in charcoal dextran stripped serum (CDSS) medium to develop castration-resistant LAPC4 (LAPC4-CR or LAPC4-AI) cells, which were subsequently exposed to either 20 M or 100 M Enza-containing CDSS medium for 1 month resulting in the LAPC4-Enza(20)-R or LAPC4-Enza(20)-R models (supplementary Fig

After treatment, the supernatant and cells were collected, washed with ice-cold 1X PBS, and stained using a dual staining method with Annexin V-FITC and propidium iodide (PI) (BioLegend, San Diego, CA, USA), according to the manufacturer's instructions

Neuroscience 172:379386 Kim YM, Pae HO, Park JE, Lee YC, Woo JM, Kim NH, Choi YK, Lee BS, Kim SR, Chung HT (2011b) Heme oxygenase in the regulation of vascular biology: from molecular mechanisms to therapeutic opportunities