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Description

Hikosaka et al

Mass spectrometry analysis of HNEylation site Following the steps described in T-REX in HEK293T cells, cell lysis (3 freezethaw) was performed in 500 l (50 mM HEPES (pH 7.6), 100 mM NaCl, 1% Nonidet P-40, 10 mM imidazole, 5 mM -mercaptoethanol and Roche protease inhibitor)