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J Am Coll Nutr 17:207215 Broad EM, Maughan RJ, Galloway SD (2005) Effects of four weeks l-carnitine l-tartrate ingestion on substrate utilization during prolonged exercise

Cancer Cell 10:375-388, 2006

Quantitative real-time PCR Quantitative real-time PCR was performed using a total reaction volume of 10 l, containing 2 l of diluted cDNA, SsoAdvanced Universal SYBR Green Supermix (1x) (Bio-Rad), and a final concentration of 0.25 M or 0.5 M of forward and reverse primers (designed using Primer-BLAST, Table S5), with a CFX Connect Real-Time Cycler and CFX manager (Bio-Rad)

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MDA-MB-231, MDA-MB-468 and MDA-MB-436 cells were seeded in 6-well plates with 10 6 /10 5 cells/well containing 3 mL media and the plates were incubated at 37 C overnight