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Western Blotting To evaluate NGF levels in the striatum and for phosphorylation levels, fresh tissue was homogenized at a 30% ratio (w v 1 ) in kinase extraction buffer A (10 mM HEPES, pH 7.9, 10 mM KCl, 10 mM EDTA, 1 mM DTT, 0.4% v v 1 IGEPAL, 1 mM Na 3 VO 4 , 1 mM PMSF, and 10 mg mL 1 aprotinin and leupeptin) using a previously described method ( g for 15 min at 4C

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The benzyl alcohol component helps inhibit bacterial growth, allowing for multiple withdrawals from a single vial when proper aseptic techniques are followed

The supernatant was incubated with glutathione Sepharose 4B (GE Healthcare) resin for 2 h

The data supporting the findings of this study are available from the corresponding authors upon reasonable request