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Resuspended samples were loaded onto equilibrated spin columns (2000 g , 2 min), passing the entire sample twice through the column

DOI: 10.1016/0009-9120(90)80046-l 43

10.1111/hel.12806 27 DorotheaMPMIPGreifenbergM

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We found that LTR had significantly higher levels of three amino acids known to increase mTOR activity in T cells (asparagine, alanine, and arginine) 14 , suggesting an extrinsic microenvironmental contribution to the increased mTOR activity seen in the CAR T cells of this cohort (Fig