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Recently, many other biological factors have been proposed to explain PE including genetic vulnerability 7, 8 , robust cortical representation of the pudendal nerve 9 , disturbances in central serotonergic neurotransmission 10, 11 , and thyroid diseases 12, 13

Harpagoside, one of its active constituents, exerts neuroprotective effects by modulating endoplasmic reticulummitochondria coupling (MAMs), suppressing IP3R1/GRP75/VDAC1 complex expression, and maintaining neuronal calcium homeostasis

We next validated these findings in human small intestinal organoids using scRNA-seq, comparing day 7 Matrigel organoids and day 4 collagen organoids, each at passage 2