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doi: 10.1097/NMD.0000000000000425 145 PfaffensellerB.Da Silva MagalhesP

Squirting water directly onto the lyophilized powder is the mistake most often blamed for foaming and degradation

Lyophilized peptides must be stored at 20C, and once reconstituted with bacteriostatic water, refrigeration at 28C maintains stability for approximately 28 days

Following treatment-induced stress or destruction, tumor cells release DAMPs such as calreticulin (CRT), 33 high mobility group box 1 (HMGB1), 34 and adenosine triphosphate (ATP), 35 which are recognized by APCs, especially dendritic cells (DCs), via PRRs, 36 leading to antigen uptake, maturation, and cross-presentation to T cells

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