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doi: 10.3390/cells10050971 25 SunJCBeilkeJNLanierLL

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Cytokine analysis, performed 8 h after administering the modified mRNA to mice with induced SP-B expression, demonstrated a 2-fold and 6-fold reduction in IFN- and IL-12 secretion, respectively, compared to unmodified mRNA

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Dithiobis(succinimidyl acetate) 56,57 SS(NHS-CM) 2 N , N -Dicyclohexylcarbodiimide (1.42 g, 6.87 mmol) was dissolved in anhydrous tetrahydrofuran (30 mL) under argon and cooled to 0 C with stirring