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One hundred and fifty microgram of the membrane fraction in 200 L membrane fraction buffer (50 mM HEPES, 10 mM potassiumacetate, 10 mM CaCl 2 , 5 mM MgCl 2 , pH = 7.5) were incubated for 1 h at 25 C under nitrogen atmosphere in the presence or absence of 5 mM L2HG or SA

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