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They may be reconstituted in a small amount of a basic solvent such as 0.1% aqueous ammonia and then diluted with water to the desired concentration

Recombinant protein purification and in vitro kinase assay Full-length human G6PD (wild-type, 2YA or 2YF mutant) sequences were cloned into the pGEX-4T1 vector and expressed as the GST-tagged fusion proteins in E

Begin with small doses, get feedback, and course correct as you go

or without these intermediate steps but also before and / or after pre-treatment and / or post-treatment steps such as acid washing, Q levels, alkaline leaching ,