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The cells harbouring the glnA gene were grown at 310 K to an OD 600 of ~0.5 in Luria-Bertani medium supplemented with 30 g mL 1 kanamycin and chloramphenicol

In advanced stages, fibrosis and adipocyte apoptosis 34 may hinder fat mobilization, paralleling findings in obesity where fibrosis impairs post-bariatric weight loss 35,36