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Free thiol assay The analyzed samples were incubated for 15 min at room temperature in 20 mM Tris-HCl buffer, pH 7.5, NaCl 100 mM, 1 mM EDTA, 5 % DMSO in the presence of 0.5 mM Ellmans Reagent (DTNB, 5,5-Dithio-bis-(2-nitrobenzoic acid), Sigma-Aldrich, Israel), and the absorbance was measured at 412 nm using the Infinite 200 PRO plate-reader (Tekan, Switzerland)

A common coupling mechanism for A-type heme-copper oxidases from bacteria to mitochondria

Both cysteine, as released in vivo from RibCys in animal models, and ribose, as administered directly to human subjects, have been found to be essentially non-toxic over wide dosage ranges

doi: 10.1577/H07-043.1 204 NawazA.Bakhsh javaidA.IrshadS.HoseinifarS

The regenerative effect of UroA was also described in a study by Guo et al