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For the targeted analysis 8 fmol of Cytochrome C digest was added to the samples before injection in LC-MS/MS (Thermo Scientific)

Hampton MB, O'Connor KM

Additionally, treatment of DIO mice with the NNMT inhibitor resulted in a substantial ~35% decrease ( P 30% decrease in adipocyte size ( P 40% decrease in adipocyte volume (data not shown) compared to control DIO mice