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Studies using the Broad Institute's Connectivity map found that GHK significantly increased the expression of DNA repair genes with 47 genes stimulated and 5 genes suppressed (more than or equal to a 50% increase or decrease) [22]

I 1 r Pf GS and I 2 r Pf GS recombinant plasmids were further subjected to site-directed mutagenesis for removing the additional amino acids that were introduced into the GS sequence due to the restriction sites used for cloning