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Since NAD cannot be taken directly from food, NAD metabolism is essential, and NAMPT is the key enzyme recovering NAD from nicotinamide and generating most of the NAD cellular pools

doi: 10.3389/FPUBH.2023.1163360 214 BahmerTBorzikowskyCLiebWHornAKristLFrickeJet al

c , The PDK1-silenced HCT116 cells stably expressing PDK1 WT or K163R were serum-starved and treated with insulin for 0 or 30 min, followed by IF with anti-Akt (red), anti-SETDB1 (green) and DAPI (blue)

Sample preparation and microscope properties are potential stress sources and can thus influence glutathione redox state and distribution within the compartments