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After 10-15 days of culture, the cells were fixed with 4% paraformaldehyde for 30 min after washing the medium with phosphate buffered saline (PBS), then stained with 0.1% crystal violet for 30 min, and the dye was washed and dried with PBS buffer, and the images were acquired after drying

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Upon ech-6 RNAi, neither consistent nor significant reduction in these transcripts were detected, largely excluding the possibility that ech-6 RNAi interferes with the transcriptional process of the neighboring genes (Fig

Instead, these larger snow globes must be checked