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JAMA Oncol 7(8):1213-1219, 2021

Membranes were blocked with Tris-buffered saline (TBS) and 0.1% Tween-20 (TBS/T) containing 5% bovine serum albumin (Sangon Biotech Co., Ltd., Shanghai, China) at 37C for 2 h, and incubated overnight at 4C with primary antibodies (Abcam, Cambridge, MA, USA

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The body can make nonessential amino acids, but it cant make essential amino acids

3.1.7 Guidelines, protocols, and recommendations The evidence in previous sections clearly suggests that exercise and physical activity benefit human health and lifespan