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Since the wild-type HGF undergoes spontaneous cleavage by the action of serum-derived proteases during the protein expression in mammalian cells, we prepared engineered HGF where the cleavage site of wild-type HGF (KQLR/V) was mutated to the recognition sequence of Factor Xa (IEGR/V)

The abundance of selected transcripts was also analyzed in gene-chip experiments

Here's why: glutathione inhibits an enzyme called tyrosinase, which plays a key role in the production of melanin (the pigment responsible for dark spots, hyperpigmentation, and uneven skin tone)

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