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Once the senescence response was established, cells were treated with 100 nM Bafilomycin (Sigma, B1793) for 1 h in the incubator, followed by the addition of 30 M C 12 FDG for 1 h in the incubator

impeded steroidogenesis (lower testosterone, follicle-stimulating hormone, luteinizing hormone, 3-Hydroxysteroid dehydrogenase(HSD), 17-HSD, and elevated cholesterol)

PMID 17227290

Measures and Assessments The following laboratory markers were analyzed before and after treatment: Plasma reduced glutathione Plasma oxidized glutathione Whole-blood glutathione Plasma cysteine Plasma taurine Plasma sulfate (total and free) All samples were collected under fasting conditions and analyzed at CLIA-certified labs