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Therefore, S1P1 negatively regulates the development of T RM cells

Western blot analysis For protein analysis, cells or pieces of liver tissue were washed in PBS and lysed in Lysis Buffer (20 mM Tris-HCl pH8, 1 mM NaCl, 0.5% NP40, 0.5% Triton-X-100, 10% glycerol) supplemented with a protease inhibitor cocktail (SigmaAldrich)

Key citation: Bhatt DL et al., Washington State University preclinical models demonstrating Dihexas HGF/c-Met-mediated synaptogenic activity

doi: 10.3390/antiox10121959 157 RenukaR.DeviK

They do, however, demonstrate this approach to be a viable strategy to be pursued as a novel oncology therapeutic avenue