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Bioinformatics analyses using the Broad Institute Connectivity Map identified GHK-Cu as a modulator of more than 4,000 human genes, with the expression signature appearing to reverse patterns associated with aging and age-related disease (PMID: 25904764)

For comparisons of more than two groups, one-way analysis of variance (ANOVA) was used to evaluate significant differences in the means and post hoc multiple comparisons were performed using the Tukey-Kramer test

There are also potential risks of thyroid tumors in animal studies, although this hasnt been conclusively proven in humans