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Reactivity of rBcp1, rC45S, rC50S toward disulfides and other thiol reagents The reactivity of sulfhydryl groups of rBcp1 toward disulfides was assayed in the following condition: protein 1.25 M final concentration, in 10 mM potassium phosphate buffer pH 7.4, 0.2 M urea (25 C), the concentrations of each disulfide were 1 mM for GSSG, cystine, cystamine, and 0.5 mM for homocystine

J Bacteriol 194:839854

Evidence also indicated a direct association between phases of migraine attacks and calcitonin gene-related peptide (CGRP), mitochondrial disorders, monoaminergic pathway, disruption in brain energy metabolism, and higher serum levels of glutamate and homocysteine

The estimation of total cellular GSH and GSSG levels was performed through GSH/GSSG-Glo Assay Kit (Promega) as per manufacturers instructions

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