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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation

(2006) Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA, EMBO J

Interestingly, SeCyS is efficiently methylated by certain plants, such as Brassica oleracea capitata (kale), Brassica juncea , and Allium tricoccum , which can accumulate high concentrations of Se in the form of selenomethionine (SeMet), resulting in increased levels of methylated metabolites [17, 18]